Hadjian A J, Culty M, Chambaz E M
Biochem Biophys Res Commun. 1984 Oct 30;124(2):393-9. doi: 10.1016/0006-291x(84)91566-3.
Addition of angiotensin II (0.3 microM) to bovine adrenal fasciculata cell suspensions prelabeled with [32P] induced a rapid (15 seconds) and marked decrease of the radioactivity from phosphatidylinositol 4,5-biphosphate (62%) and phosphatidylinositol 4-monophosphate (35%). This effect was concentration-dependent and specifically inhibited in the presence of (Sar1-Ala8)-angiotensin II; it was also completely prevented in the absence of extracellular calcium. The present data appear to illustrate the earliest biological response detectable in bovine fasciculata cells under angiotensin II challenge.
向预先用[32P]标记的牛肾上腺束状带细胞悬液中添加血管紧张素II（0.3微摩尔），导致磷脂酰肌醇4,5-二磷酸（62%）和磷脂酰肌醇4-单磷酸（35%）的放射性迅速（15秒）且显著降低。这种作用呈浓度依赖性，并且在（Sar1-Ala8）-血管紧张素II存在时受到特异性抑制；在无细胞外钙的情况下也能完全阻止这种作用。目前的数据似乎说明了在血管紧张素II刺激下牛束状带细胞中可检测到的最早生物学反应。
