Rittenhouse S E, Horne W C
Biochem Biophys Res Commun. 1984 Aug 30;123(1):393-7. doi: 10.1016/0006-291x(84)90426-1.
Human platelets exposed to ionomycin, a Ca2+ ionophore, exhibit activation of both phospholipases A2 and C. Such platelets manifest a rise in cytoplasmic Ca2+ (monitored by quin 2), a loss in phosphoinositides, formation of lysophosphatidylinositol, thromboxane B2, phosphatidic acid, and phosphorylated 47 kilodalton protein, and secretion. In the absence of thromboxane formation and secreted ADP, phospholipase C is not activated and the 47 kilodalton protein is not phosphorylated. The elevation in Ca2+ is unaffected by inhibition of cyclooxygenase and ADP. Thus, an increase in cytoplasmic Ca2+ is not sufficient to stimulate phospholipase C. Further, secretion can occur in the absence of phospholipase C activation and 47 kilodalton protein phosphorylation.
暴露于离子霉素（一种钙离子载体）的人血小板会表现出磷脂酶A2和C的激活。这类血小板表现出细胞质钙离子浓度升高（通过喹啉2监测）、磷酸肌醇减少、溶血磷脂酰肌醇、血栓素B2、磷脂酸和47千道尔顿磷酸化蛋白的形成以及分泌。在没有血栓素形成和分泌的ADP的情况下，磷脂酶C不会被激活，47千道尔顿蛋白也不会被磷酸化。钙离子浓度的升高不受环氧化酶和ADP抑制的影响。因此，细胞质钙离子浓度的升高不足以刺激磷脂酶C。此外，在没有磷脂酶C激活和47千道尔顿蛋白磷酸化的情况下也会发生分泌。
