Antibodies to a chemically synthesized peptide which precipitate a v-myb protein.

A synthetic peptide with a composition derived from the DNA sequence of v-myb of AMV was used as a hapten for immunization of rabbits. Peptide-specific immunoglobulins were isolated by affinity chromatography on a peptide-Sepharose column and used for immuno-precipitation of lysates of AMV-transformed non-producer myeloblasts. Two other proteins with molecular weights of 30 000 and 56 000 daltons apparently not related to myb protein were specifically coprecipitated with p48v-myb.