由克隆到转座子Tn5中的RK2质粒转移起始点介导的细菌染色体的接合转移。
Conjugal transfer of bacterial chromosomes mediated by the RK2 plasmid transfer origin cloned into transposon Tn5.
作者信息
Yakobson E A, Guiney D G
出版信息
J Bacteriol. 1984 Oct;160(1):451-3. doi: 10.1128/jb.160.1.451-453.1984.
Abstract
We report here a novel system for the conjugal transfer of bacterial chromosomes which utilizes the transfer origin (oriT) of plasmid RK2 cloned into transposon Tn5. Tn5 with oriT was inserted by transposition into the chromosomes of Escherichia coli and Rhizobium meliloti. The oriT sequence then served as the origin of high-frequency chromosome transfer when a helper RK2 plasmid was present in the same cell. The broad host range features of RK2 make this system of oriented chromosome mobilization applicable to most gram-negative bacteria.
摘要
我们在此报告一种用于细菌染色体接合转移的新型系统，该系统利用克隆到转座子Tn5中的质粒RK2的转移起始点（oriT）。携带oriT的Tn5通过转座插入到大肠杆菌和苜蓿根瘤菌的染色体中。当同一细胞中存在辅助RK2质粒时，oriT序列随后作为高频染色体转移的起始点。RK2的广泛宿主范围特性使得这个定向染色体动员系统适用于大多数革兰氏阴性细菌。
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