Lindwall G, Cole R D
J Biol Chem. 1984 Oct 10;259(19):12241-5.
Two newly discovered properties of tau protein are reported; it is soluble in 2.5% perchloric acid and insoluble in 25% glycerol. These properties were exploited in the development of improved methods for the purification of tau. Treatment with perchloric acid did not alter the electrophoretic behavior of tau, and the products of the new isolation method were fully competent in the promotion of microtubule assembly. The application of the new purification techniques to bovine brain tissue demonstrated that tau exists endogenously in the dephosphorylated as well as in a phosphorylated state.
据报道，τ蛋白有两个新发现的特性；它可溶于2.5%的高氯酸，而不溶于25%的甘油。这些特性被用于开发改进的τ蛋白纯化方法。用高氯酸处理不会改变τ蛋白的电泳行为，新分离方法的产物在促进微管组装方面完全有效。将新的纯化技术应用于牛脑组织表明，τ蛋白以内源性的形式存在于去磷酸化状态以及磷酸化状态。
