Connors L H, Gertz M A, Skinner M, Cohen A S
J Lab Clin Med. 1984 Oct;104(4):538-45.
Prealbumin, a negative acute-phase reactant, has a role as a clinical index of patient status after inflammation, trauma, or surgery that is gaining recognition. We developed a rate nephelometric assay that allows quantitation of prealbumin levels in a large number of samples to be performed quickly, efficiently, and reproducibly. The generation of data by this method is more rapid and precise than the alternative measurement techniques of radioimmunoassay, radial immunodiffusion, and electroimmunoassay. Nephelometric analysis of serum prealbumin in 33 patients with familial amyloidosis showed significantly depressed concentrations compared with levels in normal individuals and in patients with primary and secondary amyloidosis. Prealbumin levels were compared with corresponding amounts of the acute-phase proteins C-reactive protein and serum amyloid A in the three amyloid groups. Lowered prealbumin concentrations corresponded to elevated levels of C-reactive protein and serum amyloid A in the sera from patients with primary and secondary amyloidosis. In the group with familial amyloidosis, however, the lower than normal levels of prealbumin did not correspond to elevations in C-reactive protein and serum amyloid A. Our data suggest that depressed plasma prealbumin concentrations noted in individuals with familial amyloidosis are not simply reflections of a negative acute-phase response.
前白蛋白作为一种负急性期反应物，在炎症、创伤或手术后作为患者状态的临床指标，正逐渐得到认可。我们开发了一种速率散射比浊法，该方法能够快速、高效且可重复地对大量样本中的前白蛋白水平进行定量检测。通过这种方法生成数据比放射免疫分析、单向免疫扩散和电免疫分析等其他测量技术更快、更精确。对33例家族性淀粉样变性患者的血清前白蛋白进行散射比浊分析，结果显示与正常个体以及原发性和继发性淀粉样变性患者相比，其浓度显著降低。在这三个淀粉样变性组中，将前白蛋白水平与急性期蛋白C反应蛋白和血清淀粉样蛋白A的相应含量进行了比较。在原发性和继发性淀粉样变性患者的血清中，前白蛋白浓度降低与C反应蛋白和血清淀粉样蛋白A水平升高相对应。然而，在家族性淀粉样变性组中，前白蛋白低于正常水平与C反应蛋白和血清淀粉样蛋白A升高并不对应。我们的数据表明，家族性淀粉样变性患者中观察到的血浆前白蛋白浓度降低并非仅仅是负急性期反应的反映。
