大鼠促肾上腺皮质激素释放因子-41相关肽和血管加压素在体外灌流大鼠下丘脑促肾上腺皮质激素释放活性中的作用。

Involvement of rat corticotrophin-releasing factor-41-related peptide and vasopressin in adrenocorticotrophin-releasing activity from superfused rat hypothalami in vitro.

作者信息

Gillies G, Puri A, Hodgkinson S, Lowry P J

出版信息

J Endocrinol. 1984 Oct;103(1):25-9. doi: 10.1677/joe.0.1030025.

DOI:10.1677/joe.0.1030025

Abstract

Superfused rat hypothalamic pieces stimulated with a high K+ medium released corticotrophin-releasing factor (CRF) as detected in the rat isolated anterior pituitary cell column bioassay. This activity has components related to ovine CRF-41 (oCRF-41) and vasopressin as assessed by the effects of specific antisera on the ACTH-releasing activity of the hypothalamic column effluent. Release of immunoactive vasopressin paralleled that of CRF bioactivity. Immunoactive oCRF-41 could not be detected, as it is likely to be below the sensitivity of present radioimmunoassays.

摘要

在大鼠离体垂体前叶细胞柱生物测定中检测到，用高钾培养基刺激的大鼠下丘脑灌流切片释放促肾上腺皮质激素释放因子（CRF）。通过特异性抗血清对下丘脑柱流出物促肾上腺皮质激素释放活性的影响评估，该活性具有与绵羊CRF - 41（oCRF - 41）和血管加压素相关的成分。免疫活性血管加压素的释放与CRF生物活性的释放平行。未检测到免疫活性oCRF - 41，因为它可能低于目前放射免疫测定的灵敏度。