鸡肌酸激酶-M cDNA的分子克隆及全核苷酸序列
Molecular cloning and the complete nucleotide sequence of the creatine kinase-M cDNA from chicken.
作者信息
Kwiatkowski R W, Schweinfest C W, Dottin R P
出版信息
Nucleic Acids Res. 1984 Sep 25;12(18):6925-34. doi: 10.1093/nar/12.18.6925.
The nucleotide sequence of creatine kinase-M (CK-M) cDNA clones has been determined. It includes the entire coding region of 381 amino acids in addition to 5' and 3' untranslated regions. A comparison with a partial sequence from rat CK-M reveals 84% nucleotide sequence homology in the coding region but divergence in the 3' untranslated region. The amino acid sequence is 94% conserved between chicken and rat. Hybridization to RNA immobilized on filters indicates homology between the CK-M 3' untranslated region and additional muscle specific RNA species. The coding region hybridizes only to CK-M RNA.
已确定肌酸激酶-M（CK-M）cDNA克隆的核苷酸序列。除了5'和3'非翻译区外，它还包括381个氨基酸的完整编码区。与大鼠CK-M的部分序列比较显示，编码区的核苷酸序列同源性为84%，但3'非翻译区存在差异。鸡和大鼠之间的氨基酸序列保守性为94%。与固定在滤膜上的RNA杂交表明，CK-M 3'非翻译区与其他肌肉特异性RNA种类之间存在同源性。编码区仅与CK-M RNA杂交。
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