Tchurikov N A, Naumova A K, Zelentsova E S, Georgiev G P
Cell. 1982 Feb;28(2):365-73. doi: 10.1016/0092-8674(82)90354-3.
We have started a cloned genomic DNA fragment approximately 7 kb long (denoted as H55) from the 7B3-4 region in the X chromosome of Drosophila melanogaster. The major part of the fragment is a single-copy sequence. It directs the synthesis of mRNA that makes up approximately 0.1% of the cytoplasmic poly(A)+ RNA from Drosophila embryos. The H55 gene is split by an intervening sequence, yielding a large single-copy exon and a small repetitive 3' exon represented by hundreds of copies in the genome. This repetitive sequence ("suffix") is also present at the 3' ends of approximately 2% of all cytoplasmic poly(A) chains.
我们从黑腹果蝇X染色体的7B3 - 4区域开始研究一个长度约为7 kb的克隆基因组DNA片段（记为H55）。该片段的主要部分是单拷贝序列。它指导合成的mRNA约占果蝇胚胎细胞质多聚腺苷酸（poly(A)+）RNA的0.1%。H55基因被一个间隔序列隔开，产生一个大的单拷贝外显子和一个小的重复3'外显子，该重复外显子在基因组中以数百个拷贝的形式存在。这种重复序列（“后缀”）也存在于所有细胞质多聚腺苷酸链的约2%的3'末端。
