Zhang J L, Friedman F, Hsu K H, Specter S, Friedman H, Nonoyama M
Proc Soc Exp Biol Med. 1984 Nov;177(2):278-82. doi: 10.3181/00379727-177-41943.
Two lymphoblastoid tumor cell lines, the Burkitt lymphoma derived BJAB cell line which is free of Epstein-Barr virus (EBV) and B95-8 cells, which are marmoset lymphocytes transformed by EBV isolated from an infectious mononucleosis patient, were studied in regards to their effects on the blastogenic responsiveness of normal human peripheral blood leukocytes stimulated in vitro with mitogens. Mitomycin C treated tumor cell suspensions, when cocultured with normal human blood leukocytes, markedly depressed the expected blastogenic responses in vitro to concanavalin A, pokeweed mitogen, and phytohemagglutin. In addition, cell-free sonicates from the cell lines also depressed blastogenic responsiveness of the leukocytes in vitro. Heating the sonicates for 10 min at 100 degrees C markedly diminished the suppressive properties of the sonicates, as did ultraviolet light irradiation. The suppressive activity of the B95-8 sonicates was pelleted by high speed centrifugation as compared to the activity of sonicates derived from the BJAB cells. Further studies are warranted to determine the nature and mechanism of suppression of blastogenic responsiveness of normal human leukocytes by soluble components derived from such lymphoblastoid cell lines.
对两种淋巴母细胞瘤细胞系进行了研究，即源自伯基特淋巴瘤且不含爱泼斯坦-巴尔病毒（EBV）的BJAB细胞系，以及由从一名传染性单核细胞增多症患者分离出的EBV转化的狨猴淋巴细胞B95-8细胞系，研究它们对体外经丝裂原刺激的正常人外周血白细胞的致有丝分裂反应性的影响。经丝裂霉素C处理的肿瘤细胞悬液与正常人血白细胞共培养时，显著降低了体外对刀豆球蛋白A、商陆丝裂原和植物血凝素的预期致有丝分裂反应。此外，这些细胞系的无细胞超声裂解物也降低了白细胞体外的致有丝分裂反应性。将超声裂解物在100℃加热10分钟以及紫外线照射均显著降低了超声裂解物的抑制特性。与源自BJAB细胞的超声裂解物活性相比，B95-8超声裂解物的抑制活性经高速离心后沉淀。有必要进一步研究此类淋巴母细胞系衍生的可溶性成分抑制正常人白细胞致有丝分裂反应性的性质和机制。
