Robinson C C, Tibbetts C
Virology. 1984 Sep;137(2):276-86. doi: 10.1016/0042-6822(84)90219-8.
Repeated passage of adenovirus type 3 in HeLa cells has led to a novel stock of variant genomes, characterized by deletions and substitutions of DNA sequences within the left-end 750 base pairs. This heterogeneous stock retains few if any parental genomes--the majority of variants appear viable. Analysis of viable variants with deleted sequences reveals the 182 nucleotides proximal to the left-end inverted terminal repeat (136-318 bp) are not required for Ad3 infectivity in cultured human cell lines nor for maintenance of viral DNA encapsidation polarity.
腺病毒3型在HeLa细胞中的多次传代产生了一批新的变异基因组，其特征是左端750个碱基对内的DNA序列发生缺失和替换。这种异质群体中保留的亲本基因组极少（如果有的话）——大多数变体似乎是有活力的。对具有缺失序列的有活力变体的分析表明，靠近左端反向末端重复序列（136 - 318 bp）的182个核苷酸对于腺病毒3型在培养的人类细胞系中的感染性以及病毒DNA包装极性的维持并非必需。
