Dahlmann N, Ueckermann C
Anticancer Res. 1984 Jul-Oct;4(4-5):299-303.
A one-step micromethod was developed to separate from human serum four different enzymes capable of hydrolysing dTTP to the respective diphosphate. According to the purification procedure, the enzymes are referred to as Fraction I, II, IIIa, and IIIb, respectively. In view of a putative tumor specificity the activities of the enzymes are investigated for five different groups, epithelial carcinoma, leukemia, and three control groups. The results indicate that an absolute tumor specificity could not be attributed to one of the enzyme fractions. The activity of Fraction I is, however, highly elevated in the sera of seriously ill patients, whereas the activity of the epithelial carcinoma and the leukemia is even slightly reduced compared to the blood donor group. These data provide evidence that the determination of Fraction IIIb along with Fraction I can reduce the false positive rate to a great extent.
开发了一种一步微量法，从人血清中分离出四种不同的能够将dTTP水解为相应二磷酸的酶。根据纯化程序，这些酶分别称为组分I、II、IIIa和IIIb。鉴于假定的肿瘤特异性，对上皮癌、白血病和三个对照组这五个不同组别的酶活性进行了研究。结果表明，没有一种酶组分具有绝对的肿瘤特异性。然而，组分I的活性在重症患者血清中高度升高，而上皮癌和白血病患者血清中的活性与献血者组相比甚至略有降低。这些数据证明，同时测定组分IIIb和组分I可以在很大程度上降低假阳性率。
