Fleischmann W R, Fleischmann C M
Antiviral Res. 1984 Aug;4(4):221-8. doi: 10.1016/0166-3542(84)90020-2.
Mixed preparations of murine interferon-gamma (MuIFN-gamma) and murine interferon-alpha/beta (MuIFN-alpha/beta) have been shown to induce more than additive levels of antiviral protection, when compared to those induced by these interferons given separately. MuIFN-gamma preparations contain many lymphokines and several of these as well as MuIFN-gamma itself may participate in this potentiation. In the present study, natural as well as three recombinant DNA-derived MuIFN-gamma's, in combination with antibody to MuIFN-gamma have been employed to examine the precise role of MuIFN-gamma. The antiviral effect was examined with a single cycle virus yield reduction assay and the antiproliferative effect with a colony formation inhibition assay. Recombinant DNA-derived MuIFN-gamma was as effective as natural MuIFN-gamma at participating in the potentiation of both the antiviral and antiproliferative activities. Antibody to MuIFN-gamma effectively blocked the potentiation of both the antiviral and the antiproliferative activities of natural and recombinant DNA-derived MuIFN-gamma's. Since the recombinant DNA-derived preparations from E. colie can be assumed not to contain mammalian proteins other than MuIFN-gamma, the data conclusively demonstrate that the potentiation factor in MuIFN-gamma preparations is MuIFN-gamma itself.
与单独给予的鼠干扰素 -γ（MuIFN -γ）和鼠干扰素 -α/β（MuIFN -α/β）相比，鼠干扰素 -γ和鼠干扰素 -α/β的混合制剂已被证明能诱导出超过相加水平的抗病毒保护作用。MuIFN -γ制剂含有多种淋巴因子，其中几种以及MuIFN -γ本身可能参与了这种增强作用。在本研究中，天然的以及三种重组DNA来源的MuIFN -γ与抗MuIFN -γ抗体联合使用，以研究MuIFN -γ的确切作用。通过单循环病毒产量减少试验检测抗病毒效果，通过集落形成抑制试验检测抗增殖效果。重组DNA来源的MuIFN -γ在参与增强抗病毒和抗增殖活性方面与天然MuIFN -γ一样有效。抗MuIFN -γ抗体有效地阻断了天然和重组DNA来源的MuIFN -γ的抗病毒和抗增殖活性的增强作用。由于来自大肠杆菌的重组DNA来源制剂可以假定除了MuIFN -γ之外不含有其他哺乳动物蛋白，因此数据确凿地证明MuIFN -γ制剂中的增强因子就是MuIFN -γ本身。
