大鼠肝脏中β-肾上腺素能配体的内吞作用。内体和质膜组分中β-肾上腺素能受体与腺苷酸环化酶分布的比较。
Endocytosis of beta-adrenergic ligands by rat liver. Comparison of beta-adrenergic receptor and adenylate cyclase distribution in endosome and plasma-membrane fractions.
作者信息
Hadjiivanova N, Flint N, Evans W H, Dix C, Cooke B A
出版信息
Biochem J. 1984 Sep 15;222(3):749-54. doi: 10.1042/bj2220749.
Abstract
The internalization of beta-adrenergic receptors was investigated in rat livers perfused with an agonist ([3H]isoprenaline) or an antagonist ([125I]iodocyanopindolol). Analytical centrifugation of liver homogenates indicated that the ligands were transferred rapidly to endosomal and lysosomal positions in sucrose gradients. Endosome fractions contained beta-adrenergic binding sites, but adenylate cyclase activity was low and poorly activated by isoprenaline. The results indicate that the receptor-regulatory-protein-adenylate cyclase complex was disassembled during uptake of beta-adrenergic ligands, with the adenylate cyclase being retained at the plasma membrane.
摘要
在灌注激动剂（[3H]异丙肾上腺素）或拮抗剂（[125I]碘氰吲哚洛尔）的大鼠肝脏中研究了β-肾上腺素能受体的内化。肝脏匀浆的分析离心表明，配体在蔗糖梯度中迅速转移至内体和溶酶体位置。内体部分含有β-肾上腺素能结合位点，但腺苷酸环化酶活性较低，且异丙肾上腺素对其激活作用较弱。结果表明，在β-肾上腺素能配体摄取过程中，受体-调节蛋白-腺苷酸环化酶复合物发生了解离，腺苷酸环化酶保留在质膜上。
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