Zeytin F, Brazeau P
Biochem Biophys Res Commun. 1984 Sep 17;123(2):497-506. doi: 10.1016/0006-291x(84)90258-4.
We reported previously that GRF stimulates release of neurotensin (NT) by a clonal line of rat C cells (44-2 C). We report here that GRF stimulates calcitonin (CT) and cAMP release in these cells. For release experiments, replicate cultures are incubated for 5-180 min in serum-free defined medium. CT, NT and cAMP are measured by RIA. At a maximally effective concentration of GRF (.1-1.0 nM), there is a 2-3 fold stimulation of CT release at 60-90 min with peak release at 180 min. In contrast, GRF causes a rapid 4-6 fold increase of NT release within 5-15 min. In 44-2 C cells there is a 4-40 fold stimulation of cAMP release by GRF. We conclude that in 44-2 C cells GRF stimulates release of NT and cAMP and show for the first time the effect of this peptide on release of CT.
我们先前报道过，生长激素释放因子（GRF）可刺激大鼠C细胞克隆系（44 - 2 C）释放神经降压素（NT）。我们在此报道，GRF可刺激这些细胞释放降钙素（CT）和环磷酸腺苷（cAMP）。对于释放实验，将重复培养物在无血清限定培养基中孵育5 - 180分钟。通过放射免疫分析法（RIA）测量CT、NT和cAMP。在GRF的最大有效浓度（0.1 - 1.0 nM）下，在60 - 90分钟时CT释放受到2 - 3倍的刺激，在180分钟时释放达到峰值。相比之下，GRF在5 - 15分钟内使NT释放迅速增加4 - 6倍。在44 - 2 C细胞中，GRF可使cAMP释放受到4 - 40倍的刺激。我们得出结论，在44 - 2 C细胞中GRF可刺激NT和cAMP的释放，并且首次展示了该肽对CT释放的影响。
