McRobbie S J, Newell P C
Biochem Biophys Res Commun. 1984 Sep 28;123(3):1076-83. doi: 10.1016/s0006-291x(84)80243-0.
Dictyostelium discoideum amoebae were employed to study the refractoriness and adaptation of the rapid (5sec) accumulation of actin in their Triton-insoluble cytoskeletons following stimulation with specific chemoattractants. Amoebae became refractory within 10sec for this response but no adaptation occurred during this period. Amoebae desensitized for one attractant were not desensitized for another and responses to stimulation with a mixture of attractants were approximately additive. The characteristics of these processes are compared to published studies of adaptation in other chemoattractant-induced responses and a new model for the chemotactic signal transduction pathway is formulated. We conclude that intracellular cGMP accumulation may be on a separate branch of the pathway from the actin response.
采用盘基网柄菌变形虫来研究在用特定趋化因子刺激后，其不溶于曲通的细胞骨架中肌动蛋白快速（5秒）积累的不应性和适应性。变形虫在10秒内对此反应变得不应，但在此期间未发生适应性变化。对一种趋化因子脱敏的变形虫对另一种趋化因子并未脱敏，并且对趋化因子混合物刺激的反应大致是相加的。将这些过程的特征与已发表的关于其他趋化因子诱导反应中适应性的研究进行了比较，并构建了趋化信号转导途径的新模型。我们得出结论，细胞内cGMP积累可能位于与肌动蛋白反应不同的信号转导分支上。
