Grachev S A, Pletnev A G, Vasilenko S K, Petrov N A, Chizhikov V E
Bioorg Khim. 1983 Jul;9(7):986-9.
Reverse transcription afforded DNA-copies of the 3'-terminal sequence of mouse encephalomyocarditis virus RNA. Cloning of this cDNA in pBR 322 plasmid and sequencing of DNA of one of the clones established the structure (552 nucleotide residues) of the 3'-terminus of viral RNA. This region contains a non-translatable fragment (126 nucleotides) and also the fragment coding for the C-terminus of viral replicase.
逆转录产生了小鼠脑心肌炎病毒RNA 3'末端序列的DNA拷贝。将该cDNA克隆到pBR 322质粒中,并对其中一个克隆的DNA进行测序,确定了病毒RNA 3'末端的结构(552个核苷酸残基)。该区域包含一个不可翻译的片段(126个核苷酸)以及编码病毒复制酶C末端的片段。