Zimmermann A, Nelsen-Salz B, Kruppenbacher J P, Eggers H J
Institut für Virologie der Universität zu Köln, Federal Republic of Germany.
Virology. 1994 Sep;203(2):366-72. doi: 10.1006/viro.1994.1495.
The nucleotide sequence of a highly variant of the encephalomyocarditis virus, PV21, was determined. Discounting the poly(A) tail the viral genome is 7.861 kb in length, including a poly(C) region of 157 or 158 nucleotides. The sequence differs in 38 positions from another virulent EMCV variant, EMCV-R (1). However, the PV21 sequence shows only 85% identity with two nonlethal variants isolated in this laboratory (2). A cDNA clone covering the complete virus sequence including a poly(C) region of approximately 150 nucleotides was constructed. The recombinant virus was shown to be infectious in NMRI mice.
测定了脑心肌炎病毒高度变异株PV21的核苷酸序列。除去聚腺苷酸尾，病毒基因组长度为7.861 kb，包括一个157或158个核苷酸的聚胞嘧啶区域。该序列与另一种强毒性脑心肌炎病毒变异株EMCV-R在38个位置上不同(1)。然而，PV21序列与本实验室分离的两个非致死性变异株的同一性仅为85%(2)。构建了一个覆盖完整病毒序列的cDNA克隆，包括一个约150个核苷酸的聚胞嘧啶区域。重组病毒在NMRI小鼠中显示出传染性。
