Heath J K, Atkinson S J, Meikle M C, Reynolds J J
Biochim Biophys Acta. 1984 Nov 6;802(1):151-4. doi: 10.1016/0304-4165(84)90046-1.
Bone cells isolated from mouse calvariae by a sequential digestion procedure have many osteoblast characteristics: they respond to PTH and prostaglandin E2 by activation of adenylate cyclase but not to calcitonin, they stain for alkaline phosphatase and they make only type I collagen. In confluent monolayer culture, they do not secrete collagenase in appreciable quantities, unless stimulated with resorptive substances such as PTH, prostaglandin E2, 1,25(OH)2 vitamin D-3 and monocyte-conditioned medium. This suggests they play a direct role in bone resorption.
它们通过激活腺苷酸环化酶对甲状旁腺激素（PTH）和前列腺素E2有反应，但对降钙素无反应，它们碱性磷酸酶染色阳性，并且只产生I型胶原。在汇合的单层培养中，除非用诸如PTH、前列腺素E2、1,25(OH)2维生素D-3和单核细胞条件培养基等吸收性物质刺激，否则它们不会大量分泌胶原酶。这表明它们在骨吸收中起直接作用。
