Townsend A R, McMichael A J, Carter N P, Huddleston J A, Brownlee G G
Cell. 1984 Nov;39(1):13-25. doi: 10.1016/0092-8674(84)90187-9.
L cells expressing either the A/NT/60/68 nucleoprotein or the A/PR/8/34 (H1) hemagglutinin by DNA mediated gene transfer were used to investigate recognition by influenza A specific cytotoxic T lymphocytes (CTL). A subpopulation of CTL that recognized the H1 hemagglutinin was detected in mice primed with either A/PR/8/34 (H1N1) or A/JAP/305/57 (H2N2) influenza viruses. However, neither CTL from mice primed with A/NT/60/68 (H3N2) nor the recombinant virus X31 (H3N2) showed any activity on L cells expressing H1. These results showed that the majority of fully crossreactive CTL do not recognize the hemagglutinin molecule. A comparison between nucleoprotein and hemagglutinin transfected L cells reveals the nucleoprotein as the major target for CTL that are crossreactive on the three pandemic strains of human influenza A virus.
通过DNA介导的基因转移表达A/NT/60/68核蛋白或A/PR/8/34（H1）血凝素的L细胞，被用于研究甲型流感特异性细胞毒性T淋巴细胞（CTL）的识别情况。在用A/PR/8/34（H1N1）或A/JAP/305/57（H2N2）流感病毒免疫的小鼠中，检测到了一个识别H1血凝素的CTL亚群。然而，用A/NT/60/68（H3N2）免疫的小鼠的CTL以及重组病毒X31（H3N2)，对表达H1的L细胞均未表现出任何活性。这些结果表明，大多数完全交叉反应性CTL不识别血凝素分子。对转染了核蛋白和血凝素的L细胞进行比较发现，核蛋白是对三种甲型人流感大流行毒株具有交叉反应性的CTL的主要靶标。
