Seto S, Rabito S F, Scicli A G, Carretero O A
Circ Res. 1984 Nov;55(5):580-4. doi: 10.1161/01.res.55.5.580.
It has been reported that immunization against tonin normalizes blood pressure, and that sialoadenectomy, during which the tonin-rich salivary glands are removed, decreases blood pressure in one-kidney, one-clip hypertension. To investigate the role of tonin on this form of hypertension further, we actively immunized one-kidney, one-clip hypertensive rabbits with tonin and measured both the blood pressure response and the titer of antibodies raised against tonin. In addition, because sialoadenectomy may alter food intake, we assessed the effect of sialoadenectomy on the blood pressure of one-kidney, one-clip hypertensive rats fed a liquid diet to facilitate eating. After immunization, all rabbits developed antitonin-antibody titers ranging from 1:300 to 1:56,000. However, in none of the rabbits did the blood pressure decrease significantly (114 +/- 3 mm Hg before immunization; 129 +/- 6 mm Hg at 16 weeks after immunization). In one-kidney, one-clip hypertensive rats, sialoadenectomy did not lower blood pressure (179 +/- 5 mm Hg before sialoadenectomy; 202 +/- 9 mm Hg 3 weeks after sialoadenectomy). Neither blood pressure nor body weight differed between sialoadenectomy and sham-sialoadenectomy one-kidney, one-clip hypertensive rats (n = 6). In conclusion, neither active immunization against tonin in one-kidney, one-clip hypertensive rabbits nor sialoadenectomy in one-kidney, one-clip hypertensive rats significantly reduced established hypertension. These results do not support the hypothesis that tonin is involved in the pathogenesis of one-kidney, one-clip hypertension in these animal models.
据报道，针对高血压素进行免疫可使血压恢复正常，而切除富含高血压素的唾液腺的涎腺切除术可降低单肾单夹型高血压模型的血压。为了进一步研究高血压素在这种高血压类型中的作用，我们用高血压素对单肾单夹型高血压兔进行主动免疫，并测量血压反应以及针对高血压素产生的抗体滴度。此外，由于涎腺切除术可能会改变食物摄入量，我们评估了涎腺切除术对喂食流质饮食以方便进食的单肾单夹型高血压大鼠血压的影响。免疫后，所有兔子产生的抗高血压素抗体滴度范围为1:300至1:56,000。然而，没有一只兔子的血压显著降低（免疫前为114±3 mmHg；免疫后16周为129±6 mmHg）。在单肾单夹型高血压大鼠中，涎腺切除术并未降低血压（涎腺切除术前为179±5 mmHg；涎腺切除术后3周为202±9 mmHg）。涎腺切除术组与假手术组的单肾单夹型高血压大鼠的血压和体重均无差异（n = 6）。总之，无论是对单肾单夹型高血压兔进行针对高血压素的主动免疫，还是对单肾单夹型高血压大鼠进行涎腺切除术，都没有显著降低已形成的高血压。这些结果不支持高血压素参与这些动物模型中单肾单夹型高血压发病机制的假说。
