Simpson S R, Rahman K, Billington D
Clin Sci (Lond). 1984 Dec;67(6):647-52. doi: 10.1042/cs0670647.
Alkaline phosphodiesterase I was present in rat liver at approx. 100-fold greater activity than alkaline phosphatase, and in rat bile at approx. 25-fold greater activity. Rat serum alkaline phosphodiesterase I was increased 6-fold whilst serum alkaline phosphatase was increased only 2-fold 96 h after bile duct ligation. In contrast to alkaline phosphatase, hepatic alkaline phosphodiesterase I was not affected by bile duct ligation, suggesting its raised serum activity was due to bile regurgitation rather than overspill of the enzyme from liver into blood. Gel filtration showed that 8 and 96 h after bile duct ligation the serum contained a high molecular weight form of alkaline phosphodiesterase I. It is suggested that alkaline phosphodiesterase I offers a potentially useful indicator of biliary obstruction in the rat.
碱性磷酸二酯酶I在大鼠肝脏中的活性约比碱性磷酸酶高100倍，在大鼠胆汁中的活性约高25倍。胆管结扎96小时后，大鼠血清碱性磷酸二酯酶I增加了6倍，而血清碱性磷酸酶仅增加了2倍。与碱性磷酸酶不同，肝脏碱性磷酸二酯酶I不受胆管结扎的影响，这表明其血清活性升高是由于胆汁反流，而非酶从肝脏溢入血液。凝胶过滤显示，胆管结扎8小时和96小时后，血清中含有高分子量形式的碱性磷酸二酯酶I。有人提出，碱性磷酸二酯酶I可能是大鼠胆道梗阻的一个有用指标。
