Baldwin C L, Antczak D F, Winter A J
Dev Biol Stand. 1984;56:357-69.
The lymphocyte blastogenesis test (LBT) was evaluated for its usefulness in the diagnosis of bovine brucellosis. Using a cell titration assay which measures the blastogenic response over a range of cell concentrations, peripheral blood lymphocytes (PBL) from various populations of cattle were tested with purified Brucella abortus porin proteins and with Brucella abortus soluble antigen (BASA). Cattle tested included groups infected with virulent B. abortus strain 2308, vaccinated with B. abortus strain 19, infected with Escherichia coli strain 0116:H31 (known to cause serological cross reactions with B., abortus), vaccinated with a bacterin of Staphylococcus aureus, and unimmunized controls. Pregnant heifers vaccinated with strain 19 could generally be distinguished from pregnant animals infected with virulent strain 2308 when LBT responses to porin were analyzed by comparing maximum delta counts per minute (the responses of the cell concentration at which there is the greatest difference between stimulation in the presence of antigen and without antigen). However, a group of nonpregnant heifers infected with strain 2308 either failed to react or reacted in an inconsistent fashion with porin or BASA. The lack of responsiveness in the LBT was accompanied by high concentrations of circulating agglutinins. False positive responses also occurred to porin, although much less frequently than to the crude preparation BASA. The use of nylon wool fractionated PBL did not provide an improved means for distinguishing Brucella infected from uninfected cattle. Based on these findings, we would not recommend the LBT with either of these antigens as a routine method for diagnosis. Lipopolysaccharide was extracted from B. abortus strain 2308 and tested for its role in inducing false positive responses. Although there exist components in B. abortus extracts which are mitogenic for bovine PBL, our data do not support such a function for purified Brucella LPS.
评估了淋巴细胞增殖试验（LBT）在牛布鲁氏菌病诊断中的实用性。使用一种细胞滴定法，该方法可测量一系列细胞浓度下的增殖反应，用纯化的流产布鲁氏菌孔蛋白和流产布鲁氏菌可溶性抗原（BASA）对不同牛群的外周血淋巴细胞（PBL）进行检测。检测的牛群包括感染强毒流产布鲁氏菌2308株的组、接种流产布鲁氏菌19株的组、感染大肠杆菌0116:H31（已知会与流产布鲁氏菌产生血清学交叉反应）的组、接种金黄色葡萄球菌菌苗的组以及未免疫的对照组。当通过比较每分钟最大差值计数（存在抗原和不存在抗原时刺激之间差异最大的细胞浓度的反应）分析LBT对孔蛋白的反应时，接种19株疫苗的怀孕小母牛通常可与感染强毒2308株的怀孕动物区分开来。然而，一组感染2308株的未怀孕小母牛对孔蛋白或BASA要么无反应，要么反应不一致。LBT缺乏反应性伴随着循环凝集素的高浓度。对孔蛋白也出现了假阳性反应，尽管其频率远低于对粗制制剂BASA的反应。使用尼龙毛分离的PBL并不能提供更好的方法来区分感染布鲁氏菌和未感染布鲁氏菌的牛。基于这些发现，我们不建议将使用这两种抗原的LBT作为常规诊断方法。从流产布鲁氏菌2308株中提取脂多糖，并测试其在诱导假阳性反应中的作用。尽管流产布鲁氏菌提取物中存在对牛PBL有丝分裂原作用的成分，但我们的数据不支持纯化的布鲁氏菌脂多糖具有这种功能。
