Kaneene J M, Anderson R K, Nicoletti P, Brown R, Christenberry C C, Swann A I, Johnson D W
Am J Vet Res. 1980 Oct;41(10):1586-9.
Cell-mediated immune responses of cattle in infected herds vaccinated with Brucella abortus, strain 19, and of nonexposed controls were studied by an in vitro lymphocyte stimulation test (LST). Brucella abortus soluble antigen was used as a specific stimulator of lymphocytes. Lymphocyte suspensions were prepared from peripheral blood of cattle by the Ficoll-diatrizoate technique. Cultures were labeled with [3H]thymidine and were assayed for [3H]thymidine incorporation into DNA by liquid scintillation spectrometry. Seroagglutination tests and cultures from Brucella were conducted simultaneously with LST. Brucella abortus soluble antigen induced significantly higher (P less than or equal to 0.005) lymphocyte stimulation responses in lymphocytes from cattle infected with B abortus than in lymphocytes from cattle vaccinated with strain 19 or from nonexposed controls. There was a significant P = agreement in results of LST and seroagglutination tests in infected cattle. Among seropositive-vaccinated cattle and controls, the LST was negative.
通过体外淋巴细胞刺激试验（LST）研究了接种布鲁氏菌19号菌株的感染牛群以及未接触过布鲁氏菌的对照牛的细胞介导免疫反应。布鲁氏菌流产菌可溶性抗原用作淋巴细胞的特异性刺激物。采用聚蔗糖-泛影葡胺技术从牛的外周血制备淋巴细胞悬液。培养物用[³H]胸腺嘧啶核苷标记，并用液体闪烁光谱法测定[³H]胸腺嘧啶核苷掺入DNA的情况。同时进行布鲁氏菌血清凝集试验和培养，并与LST同步进行。布鲁氏菌流产菌可溶性抗原在感染布鲁氏菌流产菌的牛的淋巴细胞中诱导的淋巴细胞刺激反应显著高于（P≤0.005）接种19号菌株的牛或未接触过布鲁氏菌的对照牛的淋巴细胞。在感染牛中，LST和血清凝集试验的结果有显著的一致性（P = ）。在血清阳性的接种牛和对照牛中，LST为阴性。
