Krawisz J E, Sharon P, Stenson W F
Gastroenterology. 1984 Dec;87(6):1344-50.
An assay was devised to quantitate acute intestinal inflammation based on the assessment of myeloperoxidase activity. Myeloperoxidase is an enzyme found in neutrophils and, in much smaller quantities, in monocytes and macrophages. Myeloperoxidase was solubilized with hexadecyltrimethylammonium bromide and myeloperoxidase activity was measured with a dianisidine-H2O2 assay. In neutrophil suspensions, myeloperoxidase activity was directly related to cell number down to as few as 500 cells. Myeloperoxidase activity was assayed in two animal models of inflammation: acetic acid-induced colitis in rats and Clostridium difficile enterotoxin-induced enteritis in hamsters. In both models, the activity of myeloperoxidase solubilized from the inflamed tissue was directly proportional to the number of neutrophils seen in histologic sections. Histologic evaluation of neutrophil accumulation was performed by counting the number of neutrophils in a histologic section 0.18 mm long and 5 micron thick. In both animal models, myeloperoxidase activity was linearly related to neutrophil number from 400 and 4000 cells/mm. Myeloperoxidase activity from chronically inflamed colon, in which both neutrophils and histiocytes were present, was directly related to neutrophil content. Histiocytes did not contribute significantly to myeloperoxidase activity. The determination of myeloperoxidase activity in the intestine is a simple biochemical assay that can be used to quantitate inflammation.
设计了一种基于髓过氧化物酶活性评估来定量急性肠道炎症的检测方法。髓过氧化物酶是一种存在于中性粒细胞中的酶，在单核细胞和巨噬细胞中的含量则少得多。用十六烷基三甲基溴化铵溶解髓过氧化物酶，并通过二茴香胺-H2O2检测法测量髓过氧化物酶活性。在中性粒细胞悬液中，髓过氧化物酶活性与细胞数量直接相关，低至500个细胞时亦是如此。在两种炎症动物模型中检测了髓过氧化物酶活性：大鼠乙酸诱导的结肠炎和仓鼠艰难梭菌肠毒素诱导的肠炎。在这两种模型中，从炎症组织中溶解的髓过氧化物酶活性与组织学切片中可见的中性粒细胞数量成正比。通过计算0.18毫米长、5微米厚的组织学切片中的中性粒细胞数量来进行中性粒细胞积聚的组织学评估。在这两种动物模型中，髓过氧化物酶活性与每毫米400至4000个细胞的中性粒细胞数量呈线性相关。来自同时存在中性粒细胞和组织细胞的慢性炎症结肠的髓过氧化物酶活性与中性粒细胞含量直接相关。组织细胞对髓过氧化物酶活性的贡献不显著。肠道中髓过氧化物酶活性的测定是一种简单的生化检测方法，可用于定量炎症。
