Niznik H B, Burnham W M, Kish S J
J Neurochem. 1984 Dec;43(6):1732-6. doi: 10.1111/j.1471-4159.1984.tb06101.x.
The binding of [3H]flunitrazepam and [3H]RO5-4864 was measured in unwashed brain homogenates and in extensively washed brain membranes from amygdala-kindled and "yoked" control rats sacrificed 2 weeks following the sixth stage 5 convulsion. In unwashed homogenates, [3H]flunitrazepam binding was reduced in both the hypothalamus and ipsilateral right cortex of kindled rats (unchanged in other areas). In washed brain membranes, [3H]flunitrazepam binding was unaltered in these regions; it was bilaterally elevated, however, in both the amygdala and hippocampus (unchanged in other areas). In washed membranes, the in vitro addition of gamma-aminobutyric acid enhanced [3H]flunitrazepam binding to a similar extent in kindled and control membranes. These data indicate that the type of benzodiazepine binding abnormality observed after kindling depends on the type of tissue preparation employed in the assay procedure.
在第六阶段5次惊厥发作2周后处死的杏仁核点燃大鼠和“配对”对照大鼠的未洗涤脑匀浆和充分洗涤的脑膜中，测定了[³H]氟硝西泮和[³H]RO5 - 4864的结合情况。在未洗涤的匀浆中，点燃大鼠的下丘脑和同侧右皮质中[³H]氟硝西泮结合减少（其他区域无变化）。在洗涤过的脑膜中，这些区域的[³H]氟硝西泮结合未改变；然而，杏仁核和海马体中的结合在两侧均升高（其他区域无变化）。在洗涤过的脑膜中，体外添加γ-氨基丁酸在点燃和对照脑膜中以相似程度增强了[³H]氟硝西泮结合。这些数据表明，点燃后观察到的苯二氮䓬结合异常类型取决于测定过程中使用的组织制备类型。
