Young J C, Martin G S
J Virol. 1984 Dec;52(3):913-8. doi: 10.1128/JVI.52.3.913-918.1984.
We compared the intracellular location of the product of the c-fps proto-oncogene, NCP98, with that of its viral homolog P140, the transforming protein of Fujinami sarcoma virus. Using the technique of biochemical subcellular fractionation, we determined that 60 to 90% of NCP98 and its associated kinase activity are in the soluble fraction of a chicken myeloblast cell line. This fractionation behavior differs from that of P140, which is found predominantly in the particulate fraction, both in Fujinami sarcoma virus-infected chicken embryo fibroblasts and in Fujinami sarcoma virus-infected myeloblasts. The fractionation behavior of NCP98 is, however, similar to that of the P140 encoded by a temperature-sensitive strain of Fujinami sarcoma virus in infected cells grown at the nonpermissive temperature. The absence of gag sequences from NCP98 is not responsible for the difference in fractionation behavior: the v-fps transforming protein of strain F36, P91, which lacks gag sequences, is also predominantly particulate. These results indicate that association with cellular structural components correlates with the transforming activity of proteins containing fps sequences.
我们将原癌基因c-fps的产物NCP98的细胞内定位，与其病毒同源物P140（藤浪肉瘤病毒的转化蛋白）的细胞内定位进行了比较。运用生化亚细胞分级分离技术，我们确定在鸡成髓细胞系的可溶性组分中存在60%至90%的NCP98及其相关激酶活性。这种分级分离行为不同于P140，在感染藤浪肉瘤病毒的鸡胚成纤维细胞以及感染藤浪肉瘤病毒的成髓细胞中，P140主要存在于颗粒组分中。然而，NCP98的分级分离行为与在非允许温度下生长的感染细胞中，由藤浪肉瘤病毒温度敏感株编码的P140的分级分离行为相似。NCP98中不存在gag序列并非其分级分离行为差异的原因：缺乏gag序列的F36株的v-fps转化蛋白P91也主要存在于颗粒组分中。这些结果表明，与细胞结构成分的结合与含有fps序列的蛋白质的转化活性相关。
