SenGupta S, Hibi T, MacKay A, Markovic V, Stein L D, Sigal N, Gelfand E W, Chechik B E
J Natl Cancer Inst. 1984 Nov;73(5):1075-80.
The possible association of ecto-5'-nucleotidase (5'-NT) with differentiation of B-cells was explored with the use of normal and neoplastic lymphoblastoid cell lines representing sequential stages of B-cell maturation. There was no relationship between patterns of enzyme expression in the cell lines and immunoglobulin (Ig) secretion, chromosome constitution, proliferative rate, cell volume, or the presence of B1 and B2 antigens. Pre-B-cell lines, which were negative for surface Ig or Ig secretion but positive for cytoplasmic mu-chains, showed the presence of 5'-NT, whereas 9 of 11 lymphoma cell lines, Burkitt's or non-Burkitt's type, both secreting and nonsecreting, did not exhibit enzyme activity. Four myeloma cell lines and 13 of 15 normal B-cell lines were positive for 5'-NT. These results suggested that 5'-NT was present in pre-B-cells and in some very early B-cells. 5'-NT usually disappeared from early and some intermediate B-cells and reappeared in mature B-cells and plasmacytoid cells.
利用代表B细胞成熟连续阶段的正常和肿瘤性淋巴母细胞系，探讨了ecto-5'-核苷酸酶（5'-NT）与B细胞分化的可能关联。细胞系中的酶表达模式与免疫球蛋白（Ig）分泌、染色体组成、增殖率、细胞体积或B1和B2抗原的存在之间没有关系。前B细胞系表面Ig或Ig分泌呈阴性，但细胞质μ链呈阳性，显示存在5'-NT，而11个淋巴瘤细胞系中的9个，包括伯基特氏或非伯基特氏类型，无论分泌型还是非分泌型，均未表现出酶活性。4个骨髓瘤细胞系和15个正常B细胞系中的13个5'-NT呈阳性。这些结果表明，5'-NT存在于前B细胞和一些非常早期的B细胞中。5'-NT通常在早期和一些中间B细胞中消失，并在成熟B细胞和浆细胞样细胞中重新出现。
