Cohen J H, Revillard J P, Magaud J P, Lenoir G, Vuillaume M, Manel A M, Vincent C, Bryon P A
J Natl Cancer Inst. 1987 Feb;78(2):235-42.
This study addressed the possible relationship between B-cell maturation stage of Burkitt's lymphoma (BL) cell lines and Epstein-Barr virus (EBV) status, ethnic group, or type of chromosome translocation. Fifty-seven cell lines obtained at the International Agency for Research on Cancer from 51 patients were studied. Cytogenetic analyses of 54 cells lines were available. Cell size, surface immunoglobulins (sIgs), cytoplasmic immunoglobulins (cIgs), mouse red blood cell receptors, and reactivity with various monoclonal antibodies were assessed. Immunoglobulin (Ig) class secretions were measured in the supernatant of 2- and 5-day cultures from 33 cell lines, with the use of a sensitive enzyme-linked immunosorbent assay technique. From this study, BL appears to cover a broad range of the B-cell differentiation sequence, since the following Ig phenotypes were observed: null cells (sIg-, cIg-), large pre-B-cells (intracytoplasmic mu-chains), small B-cells (sIg+, cIg-), and various types of secreting B-cells (sIg+, cIg+). Among the latter, various patterns of cIg could be defined (perinuclear, paranuclear, and vesicular). B-cell maturation stages were correlated with the amount of secreted Ig. In sIg+ cell lines, different classes of Ig were found: 35 IgM, 10 IgM plus IgD, 4 IgG, and 1 IgA. None of the different monoclonal antibodies used was specific to a precise stage of maturation. The stages of maturation were correlated with neither the type of chromosome translocations of BL nor the presence of EBV genome, but the most immature cell lines were all EBV positive and most of them originated from African patients. In contrast with acute lymphoblastic leukemia, the common acute lymphocytic leukemia antigen (CD10) was expressed on nearly all BL cell lines of intermediate maturation stages but only on half of the pre-B ones. In addition, none of the cell lines tested was found to react with CD5 antibodies, which recognize most of the chronic lymphocytic leukemia of the same stage of maturation as that in the B-lymphocyte lineage.
本研究探讨了伯基特淋巴瘤（BL）细胞系的B细胞成熟阶段与爱泼斯坦-巴尔病毒（EBV）状态、种族或染色体易位类型之间的可能关系。对国际癌症研究机构从51名患者处获得的57个细胞系进行了研究。对其中54个细胞系进行了细胞遗传学分析。评估了细胞大小、表面免疫球蛋白（sIg）、细胞质免疫球蛋白（cIg）、小鼠红细胞受体以及与各种单克隆抗体的反应性。使用灵敏的酶联免疫吸附测定技术，对33个细胞系培养2天和5天的上清液中的免疫球蛋白（Ig）类别分泌情况进行了检测。从这项研究来看，BL似乎涵盖了广泛的B细胞分化序列，因为观察到了以下Ig表型：裸细胞（sIg-，cIg-）、大前B细胞（胞质内μ链）、小B细胞（sIg+，cIg-）以及各种类型的分泌性B细胞（sIg+，cIg+）。在后者中，可以定义不同的cIg模式（核周、核旁和囊泡状）。B细胞成熟阶段与分泌的Ig量相关。在sIg+细胞系中，发现了不同类别的Ig：35个IgM、10个IgM加IgD、4个IgG和1个IgA。所使用的不同单克隆抗体均不特异于精确的成熟阶段。成熟阶段与BL的染色体易位类型或EBV基因组的存在均无关联，但最不成熟的细胞系均为EBV阳性，且其中大多数源自非洲患者。与急性淋巴细胞白血病不同，常见急性淋巴细胞白血病抗原（CD10）在几乎所有处于中间成熟阶段的BL细胞系上表达，但仅在一半的前B细胞系上表达。此外，在所测试的细胞系中，未发现有细胞系与CD5抗体发生反应，而CD5抗体可识别处于与B淋巴细胞谱系相同成熟阶段的大多数慢性淋巴细胞白血病。
