Losa G A, Maestroni G J
Recent Results Cancer Res. 1984;94:276-82. doi: 10.1007/978-3-642-82295-7_30.
Thirty-seven samples of human primary breast cancer were processed for direct cloning in methylcellulose (MC) cultures. Out of the 37 specimens plated, 19 (51%) tumors grew with a plating efficiency (PE) of 0.012%. Both growing and nongrowing tumors belonged mostly to the ductal histological type. Neither the use of autologous serum (AS) nor of fetal calf serum (FCS) affected the PE. Moreover, a negative correlation was found between the level of estrogens and especially of progestin receptors and the ability of tumors to grow in MC culture. These findings underline the difficulty of cloning fresh specimens of human solid tumors and indicate that malignant cells displaying high concentrations of progestinic receptors may also display a degree of differentiation which leads to a reduced clonogenic ability.
对37份人原发性乳腺癌样本进行处理，用于在甲基纤维素（MC）培养物中直接克隆。在接种的37个标本中，19个（51%）肿瘤生长，接种效率（PE）为0.012%。生长和不生长的肿瘤大多属于导管组织学类型。使用自体血清（AS）或胎牛血清（FCS）均不影响接种效率。此外，发现雌激素尤其是孕激素受体水平与肿瘤在MC培养中的生长能力呈负相关。这些发现强调了克隆人实体瘤新鲜标本的困难，并表明显示高浓度孕激素受体的恶性细胞也可能表现出一定程度的分化，从而导致克隆能力降低。
