Moran N, Ehrenstein G, Iwasa K, Bare C, Mischke C
Science. 1984 Nov 16;226(4676):835-8. doi: 10.1126/science.6093255.
The patch-clamp technique was used to study passive movements of ions through the plasmalemma of wheat leaf protoplasts. This method overcomes the problems inherent in conventional electrophysiological study of plant cells. Changes in conductance were recorded in patches excised from the plasmalemma. Two types of patches were observed: (i) regions of low channel density, where discrete single-channel currents could be resolved and conductance ranged from 10 to 200 picosiemens and (ii) regions of high channel density, where single-channel currents could not be resolved and conductance was on the order of a few nanosiemens. The results indicate a striking similarity between animal and plant cell membranes in the basic phenomena of transport. Moreover, the approach used constitutes a new degree of refinement in the study of processes of regulation, pathology, and toxicity in plants.
采用膜片钳技术研究离子通过小麦叶原生质体质膜的被动转运。该方法克服了植物细胞传统电生理研究中固有的问题。从质膜上切除的膜片中记录到了电导变化。观察到两种类型的膜片：（i）通道密度低的区域，在该区域可分辨出离散的单通道电流，电导范围为10至200皮西门子；（ii）通道密度高的区域，在该区域无法分辨单通道电流，电导约为几纳西门子。结果表明，动物和植物细胞膜在基本转运现象上具有惊人的相似性。此外，所采用的方法在植物调节、病理学和毒性过程的研究中达到了新的精细程度。
