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植物极化细胞质膜的局域片钳：石莼根状细胞细胞壁的激光微手术。

Localized Patch Clamping of Plasma Membrane of a Polarized Plant Cell : Laser Microsurgery of the Fucus spiralis Rhizoid Cell Wall.

机构信息

Marine Biological Association, Plymouth, United Kingdom PL1 2PB.

出版信息

Plant Physiol. 1992 Aug;99(4):1686-8. doi: 10.1104/pp.99.4.1686.

DOI:10.1104/pp.99.4.1686
PMID:16669092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1080682/
Abstract

We used an ultraviolet laser to rupture a small region of cell wall of a polarized Fucus spiralis rhizoid cell and gained localized access to the plasma membrane at the growing apex. Careful control of cell turgor enabled a small portion of plasma membrane-bound cytoplasm to be exposed. Gigaohm seals allowing single-channel recordings were obtained with a high success rate using this method with conventional patch clamp techniques.

摘要

我们使用紫外激光破坏极化的石莼根状藻细胞的一小部分细胞壁，从而在生长顶端获得对质膜的局部访问。仔细控制细胞膨压可使一小部分质膜结合的细胞质暴露出来。使用这种方法结合常规的膜片钳技术，我们成功地获得了允许单通道记录的千兆欧密封。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42f7/1080682/3fe34a9f3e67/plntphys00708-0430-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42f7/1080682/3fe34a9f3e67/plntphys00708-0430-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/42f7/1080682/3fe34a9f3e67/plntphys00708-0430-a.jpg

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本文引用的文献

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