[固定在氨基有机硅色素上的禾谷镰刀菌IMV-F-1060半乳糖氧化酶的各种性质]
[Various properties of galactose oxidase from Fusarium graminearum IMV-F-1060 immobilized on aminoorganosilochromes].
作者信息
Kondakova L V, Ianishpol'skiĭ V V, Tertykh V A, Buglova T T, Koroleva O V
出版信息
Ukr Biokhim Zh (1978). 1984 Jul-Aug;56(4):394-8.
Galactose oxidase preparations are obtained from Fusarium graminearum IMV-F-N 1060 immobilized on aminoorganosilochromes activated by cyanuron chloride and 2.4-toluylene diizocyanate. The immobilized preparations were studied for their selective action on different carbohydrate substrates and for the pH-medium dependence of the obtained preparation activity. Potassium ferricyanide is established to have an activating effect on the immobilized enzyme. It is shown that the immobilized galactose oxidase preparations may be used for the analysis of galactose and lactose.
半乳糖氧化酶制剂是从禾谷镰刀菌IMV-F-N 1060中获得的，该菌固定在由三聚氯氰和2,4-甲苯二异氰酸酯活化的氨基有机硅色素上。研究了固定化制剂对不同碳水化合物底物的选择性作用以及所得制剂活性对pH介质的依赖性。已确定铁氰化钾对固定化酶有激活作用。结果表明，固定化半乳糖氧化酶制剂可用于分析半乳糖和乳糖。
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