Kondakova Lyudmila, Yanishpolskii Victor, Tertykh Valentin, Buglova Tat'yana
Institute of Surface Chemistry of National Academy of Sciences of Ukraine, Kyiv.
Anal Sci. 2007 Jan;23(1):97-101. doi: 10.2116/analsci.23.97.
Galactose oxidase from Fusarium graminearum IMV-1060 adsorbed on, and covalently bound to, silica carriers has been used for analytical determinations of D-galactose and galactose-containing sugars. Using a flowing oxygen electrode of the Clark-type, sensor system for enzymatic analysis of water solutions of galactose-containing carbohydrates was made. Measurements were taken both in the pulse and continuous modes of a substrate flowing through a column with an immobilized biocatalyst. The linear measurement ranges for galactose-containing carbohydrates concentrations were determined.
来自禾谷镰刀菌IMV - 1060的半乳糖氧化酶吸附并共价结合在二氧化硅载体上，已用于D - 半乳糖和含半乳糖糖类的分析测定。利用克拉克型流动氧电极，构建了用于含半乳糖碳水化合物水溶液酶分析的传感器系统。在底物流经固定化生物催化剂的柱的脉冲模式和连续模式下均进行了测量。测定了含半乳糖碳水化合物浓度的线性测量范围。
