Korchak H M, Roos D, Giedd K N, Wynkoop E M, Vienne K, Rutherford L E, Buyon J P, Rich A M, Weissmann G
Trans Assoc Am Physicians. 1983;96:356-64.
Neutroplasts, which are vesicles consisting of cytoplasm enclosed by plasmalemma, have been prepared and found to be incapable of degranulation in response to f-Met-Leu-Phe. However, neutroplasts generate superoxide anion in response to f-Met-Leu-Phe and PMA, and therefore, degranulation is not essential for superoxide anion generation. In addition, neutroplasts aggregate and show shape changes in response to f-Met-Leu-Phe and PMA, and thus degranulation is not essential for aggregation. Neutroplasts take up the carbocyanine dye DiOC6(3), thereby providing evidence for ionic gradients. Dye-loaded neutroplasts show fluorescence changes in response to a variety of stimuli. This response is not CN--inhibitable; therefore, activation of neutroplasts is associated with changes in membrane potential at the plasmalemma, suggesting a role for ion fluxes in the activation sequence for aggregation and superoxide anion generation.
嗜中性粒细胞胞质体是由质膜包裹细胞质形成的囊泡，已制备出该胞质体，并发现其对N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（f-Met-Leu-Phe）无脱颗粒反应。然而，嗜中性粒细胞胞质体对f-Met-Leu-Phe和佛波酯（PMA）有超氧阴离子生成反应，因此，脱颗粒对于超氧阴离子生成并非必不可少。此外，嗜中性粒细胞胞质体对f-Met-Leu-Phe和PMA有聚集反应并呈现形态变化，因此，脱颗粒对于聚集并非必不可少。嗜中性粒细胞胞质体摄取羰花青染料DiOC6(3)，从而为离子梯度提供了证据。加载染料的嗜中性粒细胞胞质体对多种刺激有荧光变化反应。这种反应不受氰化物抑制；因此，嗜中性粒细胞胞质体的激活与质膜处膜电位的变化相关，提示离子通量在聚集和超氧阴离子生成的激活序列中起作用。
