虎皮鹦鹉幼雏病病毒的研究
Investigations of budgerigar fledgling disease virus.
作者信息
Dykstra M J, Dykstra C C, Lukert P D, Bozeman L H
出版信息
Am J Vet Res. 1984 Sep;45(9):1883-7.
PMID:6093653
Abstract
The DNA of a papovavirus associated with budgerigar fledgling disease was purified and cloned into plasmid pBR322. The genome was circular and approximately 5.1 kilobases long. Physical mapping of the genome with restriction endonucleases revealed little similarity to simian virus 40 or polyomavirus DNA. Transformation trials using murine 3T3 cells were negative. Attempts to characterize proteins were unsuccessful due to the apparent close association of the virus and host cell components.
摘要
与虎皮鹦鹉雏鸟病相关的乳头瘤多瘤空泡病毒的DNA被纯化并克隆到质粒pBR322中。该基因组呈环状，长度约为5.1千碱基。用限制性内切酶对基因组进行物理图谱分析，结果显示与猴病毒40或多瘤病毒DNA几乎没有相似性。使用鼠3T3细胞进行的转化试验结果为阴性。由于病毒与宿主细胞成分明显紧密相关，对蛋白质进行表征的尝试未成功。
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