Deffie A M, LéJohn H B
Biochem Biophys Res Commun. 1984 Oct 15;124(1):6-13. doi: 10.1016/0006-291x(84)90908-2.
Mammalian cells transformed with either 9,10-dimethyl-1,2-benzanthracene, SV40 or H-ras oncogene dramatically changed their ability to synthesize DNA and RNA and metabolize polyphosphate when L-glutamine was withdrawn from the growth medium or when heat shocked (growth at 42 degrees C). Untransformed, DNA and RNA synthesis decreased by 50-80% when glutamine was withdrawn, but polyphosphate accumulated whether or not glutamine was supplied. Heat shock did not alter this response. Transformed isogenic cells responded differently; at 37 degrees C, they decreased their synthesis of DNA and RNA if starved for glutamine, whereas at 42 degrees C, synthesis was optimal without glutamine. Transformed cells accumulated polyphosphate at 37 degrees C when starved for glutamine, but at 42 degrees C, no polyphosphate accumulated. This apparent non-dependence on glutamine by transformed cells when heat shocked was found to be due to the production of glutamine from serum proteins through induction of a protease(s).
用9,10 - 二甲基 - 1,2 - 苯并蒽、SV40或H - ras癌基因转化的哺乳动物细胞，当从生长培养基中去除L - 谷氨酰胺或进行热休克处理（在42℃生长）时，其合成DNA和RNA以及代谢多聚磷酸盐的能力会发生显著变化。未转化的细胞，去除谷氨酰胺时DNA和RNA合成减少50 - 80%，但无论是否供应谷氨酰胺，多聚磷酸盐都会积累。热休克并未改变这种反应。转化的同基因细胞反应不同；在37℃时，如果缺乏谷氨酰胺，它们会减少DNA和RNA的合成，而在42℃时，缺乏谷氨酰胺时合成最为 optimal。缺乏谷氨酰胺时，转化细胞在37℃会积累多聚磷酸盐，但在42℃时不会积累多聚磷酸盐。发现转化细胞在热休克时对谷氨酰胺的这种明显不依赖性是由于通过诱导一种或多种蛋白酶从血清蛋白中产生了谷氨酰胺。
