大鼠胶原酶分离胰岛及长期复合胰岛同种异体移植的观察

Millard P R, Reece-Smith H, Smart Y C, McShane P, Morris P J

Br J Exp Pathol. 1984 Dec;65(6):745-51.

Collagenase digest preparations of isolated rat islets and long-surviving allogeneic rat islets implanted beneath the kidney capsule of a composite kidney and islet allograft have been studied immunocytochemically and by electron microscopy. The four main endocrine cell types were identified in the collagenase preparations with minimal evidence of cell damage. In contrast, there were only granulated B cells in the composite grafts and amongst the granules of these cells there was pleomorphism. The granule appearances may support previous suggestions that paracrine control of insulin secretion is lacking in these composite grafts.

对分离的大鼠胰岛的胶原酶消化制剂以及植入复合肾和胰岛同种异体移植肾被膜下的长期存活的同种异体大鼠胰岛进行了免疫细胞化学和电子显微镜研究。在胶原酶制剂中鉴定出四种主要的内分泌细胞类型，细胞损伤迹象极小。相比之下，复合移植物中仅存在颗粒状B细胞，并且在这些细胞的颗粒中存在多形性。颗粒外观可能支持先前的观点，即这些复合移植物中缺乏胰岛素分泌的旁分泌控制。