Taniguchi N, Miyawaki T, Yachie A, Ikuta N, Ohzeki S
Diagn Immunol. 1983;1(3):104-11.
The in vivo expression of T-cell activation antigens defined by means of an OKT series of monoclonal antibodies and the anti-Tac antibody was evaluated in peripheral T lymphocytes obtained from patients with infectious mononucleosis and in cerebrospinal fluid (CSF) cells collected from individuals with mumps meningitis by the immunoperoxidase method. Although Tac antigen was definitely expressed on about 10% of CSF cells in mumps meningitis, Tac+ T cells could not be identified on any peripheral blood T cells in infectious mononucleosis, which expressed OKIa 1+, OKT10+, and OKT9+ determinants, as a probable sign of in vivo activation. The coexpression of Ia+ antigens on a T-cell functional subset might be important for their functional properties. Our kinetic study and cell cycle analysis of the expression of these activation antigens suggested that Tac antigen might be expressed at the early stage of the G1 phase of the cell cycle; OKT9+ antigen at the late stage of the G1; and Ia+ and OKT10+ determinants might be expressed at the S phase or later on in vitro, probably one after another.
采用免疫过氧化物酶法，通过OKT系列单克隆抗体和抗Tac抗体来定义，评估传染性单核细胞增多症患者外周血T淋巴细胞以及腮腺炎脑膜炎患者脑脊液（CSF）细胞中T细胞活化抗原的体内表达情况。尽管在腮腺炎脑膜炎患者中，约10%的CSF细胞确实表达Tac抗原，但在传染性单核细胞增多症患者的任何外周血T细胞上均未识别出Tac+ T细胞，而这些外周血T细胞表达OKIa 1+、OKT10+和OKT9+决定簇，这可能是体内活化的一个迹象。T细胞功能亚群上Ia+抗原的共表达可能对其功能特性很重要。我们对这些活化抗原表达的动力学研究和细胞周期分析表明，Tac抗原可能在细胞周期G1期早期表达；OKT9+抗原在G1期晚期表达；Ia+和OKT10+决定簇可能在S期或体外更晚阶段表达，可能是相继表达。
