Lemm G, Warnatz H
Clin Exp Immunol. 1986 Apr;64(1):71-9.
Synovial fluid lymphocytes (SFL) and peripheral blood lymphocytes (PBL) from patients with rheumatoid arthritis (RA) and reactive oligoarthritis were investigated for activated T cells (Ia+SIg-), IL-2 receptor bearing cells (Tac+) and IL-2 production in vivo and in vitro. In contrast to negative results with blood, the synovial fluid of the arthritic joints contains considerable amounts of IL-2 activity (median: 11.8 mu/ml), elevated proportions of Ia+SIg- activated T cells (median: 12.5%) and of IL-2 receptor bearing cells (median: 2.5%). In vitro, after stimulation with several Concanavalin A (Con A) doses, SFL develop proportions of IL-2 receptor cells comparable to PBL. Furthermore, they produce higher values of IL-2 activity than comparable PBL cultures. The proportions of Ia+SIg- activated T cells increase only moderately after Con A stimulation compared to in vivo data, indicating different activated T cell subsets in the synovial fluid (Ia+SIg-, Tac+). The findings are discussed as an expression of an acute hyperactivation of lymphocytes in an inflamed joint.
对类风湿性关节炎（RA）和反应性寡关节炎患者的滑液淋巴细胞（SFL）和外周血淋巴细胞（PBL）进行了研究，以检测体内和体外活化T细胞（Ia + SIg -）、携带白细胞介素-2受体的细胞（Tac +）以及白细胞介素-2的产生情况。与血液检测的阴性结果相反，关节炎关节的滑液含有相当数量的白细胞介素-2活性（中位数：11.8μ/ml）、比例升高的Ia + SIg -活化T细胞（中位数：12.5%）和携带白细胞介素-2受体的细胞（中位数：2.5%）。在体外，用几种剂量的伴刀豆球蛋白A（Con A）刺激后，SFL产生的白细胞介素-2受体细胞比例与PBL相当。此外，它们产生的白细胞介素-2活性值高于相应的PBL培养物。与体内数据相比，Con A刺激后Ia + SIg -活化T细胞的比例仅适度增加，表明滑液中存在不同的活化T细胞亚群（Ia + SIg -、Tac +）。这些发现被认为是炎症关节中淋巴细胞急性过度活化的一种表现。
