Villa R F, Gorini A
Int J Clin Pharmacol Res. 1984;4(4):263-75.
Changes in the maximal rate of some enzymatic activities related to energy transduction (lactate dehydrogenase; citrate synthetase and malate dehydrogenase; total NADH-cytochrome c reductase and cytochrome oxidase) and others such as glutamate dehydrogenase and acetylcholine esterase were assayed both in the purified mitochondrial fraction and in the crude synaptosomal fraction from the cerebral cortex of rats. The evaluations were performed before and after a postdecapitative normothermic ischaemia of 5, 10, 20 and 40 min duration. The ischaemic damage resulted in a decrease in the activity of mitochondrial malate dehydrogenase and total NADH-cytochrome c reductase, and of synaptosomal acetylcholine esterase. The biochemical evaluations were performed also after an intraperitoneal pretreatment with vincamine TPS, trimetazidine DC and suloctidil (50 mg/kg). These drugs induced different changes in enzyme activities as a function of the duration of ischaemia. These various interferences are discussed with regard to the possible mode of action of the drugs.
对大鼠大脑皮质纯化线粒体部分和粗制突触体部分中，一些与能量转导相关的酶活性（乳酸脱氢酶、柠檬酸合成酶和苹果酸脱氢酶、总NADH - 细胞色素c还原酶和细胞色素氧化酶）以及其他酶如谷氨酸脱氢酶和乙酰胆碱酯酶的最大反应速率变化进行了测定。在持续5、10、20和40分钟的断头后常温缺血前后进行了评估。缺血损伤导致线粒体苹果酸脱氢酶和总NADH - 细胞色素c还原酶以及突触体乙酰胆碱酯酶的活性降低。在用长春胺TPS、曲美他嗪DC和舒洛地尔（50mg/kg）进行腹腔预处理后也进行了生化评估。这些药物根据缺血持续时间诱导了酶活性的不同变化。针对药物可能的作用方式对这些不同的干扰进行了讨论。
