Dagani F, Gorini A, Polgatti M, Villa R F, Benzi G
J Neurosci Res. 1983;10(2):135-40. doi: 10.1002/jnr.490100203.
In order to investigate the in vivo pharmacological effects of the drug naftidrofuryl, we prepared populations of synaptic and nonsynaptic mitochondria from rat brain cortex. In these different mitochondrial populations the activities of citrate synthase, malate dehydrogenase, total NADH cytochrome c reductase, cytochrome oxidase, and glutamate dehydrogenase were evaluated. Except for glutamate dehydrogenase, the specific activities of the enzymes evaluated in the "free" mitochondrial fraction were higher than those observed in the "synaptic" SM1 and SM2 mitochondrial fractions, the difference between SM1 and SM2 fractions being significant. The in vivo administration of naftidrofuryl induced few and different changes in the various mitochondrial populations.
为了研究萘呋胺酯药物的体内药理作用，我们从大鼠脑皮质制备了突触线粒体和非突触线粒体群体。在这些不同的线粒体群体中，评估了柠檬酸合酶、苹果酸脱氢酶、总NADH细胞色素c还原酶、细胞色素氧化酶和谷氨酸脱氢酶的活性。除谷氨酸脱氢酶外，在“游离”线粒体部分评估的酶的比活性高于在“突触”SM1和SM2线粒体部分观察到的比活性，SM1和SM2部分之间的差异显著。萘呋胺酯的体内给药在各种线粒体群体中引起了很少且不同的变化。
