Ishiguro N, Sato G
J Bacteriol. 1984 Nov;160(2):642-50. doi: 10.1128/jb.160.2.642-650.1984.
The citrate utilization (Cit+) transposon Tn3411 was shown to be flanked by directly repeated sequences (IS3411L and IS3411R) by restriction enzyme analysis and electron microscope observation. Cit- deletion mutants were frequently found to be generated in pBR322::Tn3411 by intramolecular recombination between the two copies of IS3411. The flanking IS3411 elements of Tn3411 were shown to be functional insertion sequences by Tn3411-mediated direct and inverse transposition. Tn3411-mediated inverse transposition from pBR322::Tn3411 to the F-plasmid derivative pED100 occurred more efficiently than that of direct transposition of the Cit+ determinant. This was thought to be due to the differential transposability of IS3411L and IS3411R in the transposition process. The frequency of transposition of IS3411 marked with a chloramphenicol resistance determinant was much higher than IS3411-mediated cointegrate formation, suggesting that replicon fusions are not essential intermediates in the transposition process of Tn3411 or IS3411. Spontaneous deletions occurred with high frequency in recA hosts. The spontaneous deletion promoted by homologous recombination between two IS3411 elements in Tn3411 was examined with deletion mutants.
通过限制性内切酶分析和电子显微镜观察表明，柠檬酸利用（Cit +）转座子Tn3411两侧存在直接重复序列（IS3411L和IS3411R）。在pBR322::Tn3411中，通过IS3411的两个拷贝之间的分子内重组，经常发现产生Cit - 缺失突变体。通过Tn3411介导的直接和反向转座，表明Tn3411的侧翼IS3411元件是功能性插入序列。Tn3411介导的从pBR322::Tn3411到F质粒衍生物pED100的反向转座比Cit + 决定簇的直接转座更有效。这被认为是由于IS3411L和IS3411R在转座过程中的转座能力不同。用氯霉素抗性决定簇标记的IS3411的转座频率远高于IS3411介导的共整合体形成，这表明复制子融合不是Tn3411或IS3411转座过程中的必需中间体。在recA宿主中，自发缺失高频发生。用缺失突变体检测了Tn3411中两个IS3411元件之间同源重组促进的自发缺失。
