Ishiguro N, Sato G
J Bacteriol. 1985 Dec;164(3):977-82. doi: 10.1128/jb.164.3.977-982.1985.
The citrate utilization determinant from transposon Tn3411 has been cloned and sequenced, and its polypeptide products have been characterized in minicell experiments. The nucleotide sequence was determined for a 2,047-base-pair BglII restriction endonuclease fragment that includes the citrate determinant. This region contains an open reading frame that would encode a 431-amino-acid very hydrophobic polypeptide and which is preceded by a reasonable ribosomal binding site. However, the single polypeptide found in minicell experiments had an apparent molecular weight of 35,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
来自转座子Tn3411的柠檬酸盐利用决定簇已被克隆和测序，其多肽产物已在小细胞实验中得到表征。测定了一个包含柠檬酸盐决定簇的2047个碱基对的BglII限制性内切酶片段的核苷酸序列。该区域包含一个开放阅读框，它将编码一个431个氨基酸的非常疏水的多肽，并且在其前面有一个合理的核糖体结合位点。然而，在小细胞实验中发现的单一多肽在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳上的表观分子量为35000。
