Vethamany-Globus S, Globus M, Milton G
J Exp Zool. 1984 Nov;232(2):259-67. doi: 10.1002/jez.1402320213.
Plasma beta-endorphin (beta-EP) levels were measured at different stages of metamorphosis in anuran species, namely Rana catesbeiana, R. pipiens, and Xenopus laevis. The mean levels of beta-EP in tadpoles and postmetamorphic juveniles were significantly higher than adult levels in all three species examined. The pattern of high tadpole levels and low adult levels corresponds with the limb regeneration ability of the tadpole and restriction in the adult. We have also reported plasma beta-EP levels in two urodele species, Ambystoma tigrinum and A. jeffersonianum. Localization of beta-EP by immunocytochemistry shows bright fluorescence to beta-EP antiserum in the wound epithelium of the regeneration blastema. The mesoderm and nuclei of all blastema cells remain nonreactive. It is suggested that receptors to beta-EP reside in the epidermis.
在蛙类物种（即牛蛙、豹蛙和非洲爪蟾）变态发育的不同阶段测量了血浆β-内啡肽（β-EP）水平。在所研究的所有三个物种中，蝌蚪和变态后的幼体中β-EP的平均水平显著高于成体水平。蝌蚪水平高而成体水平低的模式与蝌蚪的肢体再生能力和成体的限制相对应。我们还报告了两种蝾螈物种（虎纹钝口螈和杰斐逊钝口螈）的血浆β-EP水平。通过免疫细胞化学对β-EP进行定位显示，再生芽基的伤口上皮中β-EP抗血清呈现明亮荧光。所有芽基细胞的中胚层和细胞核均无反应。提示β-EP的受体存在于表皮中。
