从源自人直肠癌的分化上皮细胞系（HRT - 18）的粪便样本中分离和鉴定肠道病毒。
Isolation and identification of enteroviruses from faecal samples in a differentiated epithelial cell line (HRT-18) derived from human rectal carcinoma.
作者信息
Patel J R, Daniel J, Mathan M, Mathan V I
出版信息
J Med Virol. 1984;14(3):255-61. doi: 10.1002/jmv.1890140309.
Abstract
A human rectal carcinoma-derived differentiated epithelial cell line, HRT-18, was inoculated with faecal samples in an attempt to grow coronavirus-like particles (CVLP), which are widely prevalent in human stools in southern India. While CVLP did not grow in this cell line, a variety of enteroviruses were isolated from 48 of the 114 stool samples from healthy controls and patients with diarrhoea. The results suggest that human gastrointestinal tumour-derived differentiated epithelial cells in continuous culture may be useful for the primary isolation of enteroviruses and merit further study.
摘要
将源自人直肠癌的分化上皮细胞系HRT-18接种粪便样本，试图培养冠状病毒样颗粒（CVLP），这种颗粒在印度南部人类粪便中广泛存在。虽然CVLP在该细胞系中未生长，但从114份健康对照和腹泻患者的粪便样本中的48份中分离出了多种肠道病毒。结果表明，连续培养的源自人胃肠道肿瘤的分化上皮细胞可能有助于肠道病毒的初次分离，值得进一步研究。
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