枯草芽孢杆菌染色体末端区域复制叉移动的障碍。

Impediment to replication fork movement in the terminus region of the Bacillus subtilis chromosome.

作者信息

Weiss A S, Wake R G

出版信息

J Mol Biol. 1984 Nov 15;179(4):745-50. doi: 10.1016/0022-2836(84)90165-7.

Abstract

The terminus regions of the chromosomes of three strains of Bacillus subtilis 168 were radioactively labelled by supplying [3H]thymine towards the end of a round of replication. These strains lacked or contained the prophage SP beta c2. Following restriction endonuclease digestion of the purified DNA and fluorography, an SP beta c2-related perturbation of the terminus-labelling profile was observed, which was completely consistent with the previously suggested existence of an impediment to replication fork movement (terC) within a BamHI 24.8 X 10(3) base fragment (Weiss & Wake, 1983). The present data suggest that terC is located within the 11.4 X 10(3) base BamHI + SalI double-digest portion of this BamHI fragment.

摘要

通过在一轮复制接近尾声时提供[3H]胸腺嘧啶，对三株枯草芽孢杆菌168染色体的末端区域进行放射性标记。这些菌株缺乏或含有原噬菌体SPβc2。在对纯化的DNA进行限制性内切酶消化和荧光自显影后，观察到与末端标记图谱相关的SPβc2扰动，这与之前提出的在BamHI 24.8×10³碱基片段内存在复制叉移动障碍（terC）完全一致（Weiss和Wake，1983年）。目前的数据表明，terC位于该BamHI片段11.4×10³碱基的BamHI + SalI双酶切部分内。