Hansen E B, Atlung T, Hansen F G, Skovgaard O, von Meyenburg K
Mol Gen Genet. 1984;196(3):387-96. doi: 10.1007/BF00436184.
A fine structure genetic map of several mutations in the dnaA gene of Escherichia coli was constructed by the use of recombinant lambda and M13 phages. The dnaA508 mutation was found to be the mutation most proximal to the promoter, while the dnaA203 mutation was found to be the most distal one. The order of mutations established in this analysis was: dnaA508, dnaA167, (dnaA5, dnaA46, dnaA211), dnaA205, dnaA204, dnaA203. The mutations dnaA601, dnaA602, dnaA603, dnaA604 and dnaA606 were found to map very close to each other and close to dnaA205 in the middle third of the dnaA gene. In analysing the dominance relationship all 13 dnaA mutations were found to be recessive to the wild type. Characteristic phenotypes of the dnaA(Ts) mutants, like reversibility of the temperature inactivation of the dnaA protein, cold sensitivity of haploid or of merodiploid strains and suppressibility by rpoB mutations, are found to correlate with clusters of mutations within the gene.
利用重组λ噬菌体和M13噬菌体构建了大肠杆菌dnaA基因中几个突变的精细结构遗传图谱。发现dnaA508突变是最靠近启动子的突变，而dnaA203突变是最远离启动子的突变。在该分析中确定的突变顺序为：dnaA508、dnaA167、(dnaA5、dnaA46、dnaA211)、dnaA205、dnaA204、dnaA203。发现突变dnaA601、dnaA602、dnaA603、dnaA604和dnaA606在图谱上彼此非常靠近，且在dnaA基因中间三分之一区域靠近dnaA205。在分析显性关系时，发现所有13个dnaA突变相对于野生型都是隐性的。发现dnaA(Ts)突变体的特征表型，如dnaA蛋白温度失活的可逆性、单倍体或部分二倍体菌株的冷敏感性以及被rpoB突变抑制，与基因内的突变簇相关。
