Nedospasov S A, Shakhov A N, Georgiev G P
Mol Biol (Mosk). 1984 Jul-Aug;18(4):1099-110.
Micrococcal nuclease digestion and hybridization end labeling procedure have been used for analysis of nucleosomal organization of SV40 minichromosomes. Usual oligonucleosomal pattern containing long oligonucleosomes has been observed after digestion and DNA electrophoresis. All the regions of the minichromosome, including the "regulatory" one, are involved in nucleosome structure, as judged by hybridization analysis. This is valid at least for the major minichromosome fraction. On the other hand, the nucleosome ordering turned out to be higher in certain genome regions, for example around the site where the replication terminates. Data implying the existence of several discrete nucleosome "frames" in this region have been obtained. Possible artefacts due to micrococcal nuclease sequence-specificity are discussed.
微球菌核酸酶消化和杂交末端标记程序已用于分析SV40微型染色体的核小体组织。消化和DNA电泳后观察到含有长核小体的常见寡核小体模式。通过杂交分析判断，微型染色体的所有区域，包括“调控”区域，都参与了核小体结构。这至少对主要的微型染色体部分是有效的。另一方面，在某些基因组区域，例如复制终止位点周围，核小体的排列更为有序。已获得暗示该区域存在几个离散核小体“框架”的数据。讨论了由于微球菌核酸酶序列特异性可能产生的假象。
