Blake M J, Stein E A, Vomachka A J
Peptides. 1984 Sep-Oct;5(5):953-8. doi: 10.1016/0196-9781(84)90122-0.
In order to investigate the effects of long-term exercise training on brain endorphin systems, and the latter's possible effects on the hypothalamic-pituitary-gonadal axis, female Wistar rats were subjected to daily treadmill running. A sedentary control group was also employed. After 8 weeks of training, and just prior to sacrifice, one-half of each group received a final fatiguing bout of exercise. Thus the final four groups consisted of a trained-fatigued (TF), trained-nonfatigued (TN), control-fatigued (CF), and control-nonfatigued (CN) group. Regional brain levels of beta-endorphin (beta E), methionine enkephalin and leucine enkephalin (LE) were assayed with independent RIAs from the nucleus accumbens, cortex, caudate-putamen, septum, amygdala, anterior and posterior hypothalamus, substantia nigra and ventral tegmentum. Diestrus serum levels of luteinizing hormone (LH), follicle stimulating hormone and prolactin (PRL) were also determined. Fatiguing resulted in a decrease in serum LH levels as well as an increase in beta E content in the nucleus accumbens, and LE content in the ventral tegmentum. Finally, TF animals exhibited less LE in the amygdala than the TN rats. Taken together, these changes in brain endorphins may indicate an acute, fatigue-running modulation of the hypothalamic-pituitary-gonadal axis.
为了研究长期运动训练对脑内啡系统的影响，以及后者对下丘脑 - 垂体 - 性腺轴的可能作用，对雌性Wistar大鼠进行每日跑步机跑步训练。同时设立了久坐不动的对照组。训练8周后，在处死前，每组中的一半大鼠进行了最后一次疲劳性运动。因此，最终的四组包括训练疲劳组（TF）、训练非疲劳组（TN）、对照疲劳组（CF）和对照非疲劳组（CN）。采用独立的放射免疫分析法检测了伏隔核、皮质、尾状核 - 壳核、隔区、杏仁核、下丘脑前区和后区、黑质和腹侧被盖区的脑内β - 内啡肽（βE）、甲硫氨酸脑啡肽和亮氨酸脑啡肽（LE）水平。还测定了动情间期血清促黄体生成素（LH）、促卵泡激素和催乳素（PRL）水平。疲劳导致血清LH水平降低，伏隔核中βE含量增加，腹侧被盖区中LE含量增加。最后，TF组动物杏仁核中的LE含量低于TN组大鼠。综上所述，脑内啡的这些变化可能表明下丘脑 - 垂体 - 性腺轴存在急性的、由疲劳跑步引起的调节作用。
